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目的建立高效液相法(HPLC)测定当归补血丸中阿魏酸含量的方法。方法采用HPLC测定当归补血丸中的阿魏酸含量,C18柱:4.6 mm×250 mm,5μm;流动相采用甲醇-1.0%冰醋酸溶液。结果阿魏酸在5.00~80.00μg/ml浓度范围与峰面积具有良好的线性关系(r=0.9998),回收率为98.86%,RSD=1.13%。结论采用HPLC测定当归补血丸中的阿魏酸含量具有简便、准确和灵敏的优势,可用于对当归补血丸进行质量控制。
Objective To establish a method for the determination of ferulic acid in Dangguibuxuewan by HPLC. Methods The content of ferulic acid in Dangguibuxue Pill was determined by HPLC. The column of C18 was 4.6 mm × 250 mm, 5 μm. The mobile phase was methanol - 1.0% acetic acid solution. Results Ferulic acid showed a good linearity (r = 0.9998) with a peak area of 5.00 ~ 80.00μg / ml with a recovery of 98.86% and a RSD of 1.13%. Conclusion The determination of ferulic acid in Dangguibuxue Pill by HPLC is simple, accurate and sensitive. It can be used for quality control of Dangguibuxue Pill.