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目的:探讨大肠癌原发灶及其肝转移灶组织Fas、FasL蛋白的表达比较及其意义。方法:应用S-P免疫组化法检测30例大肠癌原发灶及其肝转移灶组织、20例正常肠黏膜组织中Fas、FasL的表达情况。结果:大肠癌原发灶和肝转移灶的Fas表达率显著低于正常组织中的表达,但原发灶与转移灶之间差别不显著。原发灶和肝转移灶的FasL表达率分别为63.33%和86.67%。显著高于正常组织中的表达,转移灶瘤细胞FasL表达率高于原发灶中的表达,二者差别具有统计学意义(P<0.05)。原发灶和转移灶中TILs均可测得Fas、FasL不同程度的表达。结论:FasL在大肠癌肝转移中起主要作用,瘤细胞FasL通过Fas-FasL介导凋亡消灭肿瘤浸润淋巴细胞,为其自身在肝脏建群生长提供空间、逃避免疫系统监视,促进转移过程顺利进行。
Objective: To investigate the expression and significance of Fas and FasL proteins in primary and metastatic colorectal cancer. Methods: The expression of Fas and FasL in 30 cases of primary colorectal cancer and its metastatic liver tissue and 20 cases of normal intestinal mucosa were detected by S-P immunohistochemistry. Results: The expression of Fas in primary and hepatic metastases of colorectal cancer was significantly lower than that in normal tissues, but the difference between primary and metastatic lesions was not significant. FasL expression rates in primary and hepatic metastases were 63.33% and 86.67%, respectively. Which was significantly higher than that in normal tissues. The expression of FasL in metastatic tumor cells was higher than that in primary tumor, the difference was statistically significant (P <0.05). TILs in primary tumor and metastasis can be detected in different degrees of expression of Fas and FasL. Conclusion: FasL plays a major role in hepatic metastasis of colorectal cancer. FasL induces apoptosis via Fas-FasL and abolishes tumor-infiltrating lymphocytes, providing space for self-colonization in the liver, avoiding immune system surveillance and facilitating the metastasis get on.