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目的探讨zeste基因增强子同源物2(EZH2)mRNA在结直肠癌组织中的表达及其与结直肠癌临床病理特征的关系。方法采用PCR方法检测了27例结直肠癌患者的癌组织、癌旁组织和正常黏膜上皮组织中EZH2 mRNA的表达情况,并分析其与结直肠癌临床病理特征的关系。结果结直肠癌组织、癌旁组织及正常黏膜上皮组织中EZH2 mRNA的表达水平分别为3.01±1.29、1.20±0.69及0.87±0.37,其在结直肠癌组织的表达水平最高(P<0.01),而在癌旁组织和正常黏膜上皮组织中的表达差异无统计学意义(P=0.403)。结直肠癌组织中EZH2 mRNA的表达与其TNM分期(P=0.002)和组织分化程度(P=0.005)均有关,与年龄(P=0.388)、性别(P=0.963)、肿瘤直径(P=0.579)、组织学类型(P=0.945)、肿瘤位置(P=0.611)及淋巴结转移(P=0.449)均无关。结论 EZH2 mRNA在结直肠癌组织中呈高表达,可能在结直肠癌的发生和发展过程中起关键作用,可作为判断结直肠癌恶性程度及进程的参考指标之一。
Objective To investigate the expression of zeste gene enhancer homolog 2 (ERZH2) mRNA in colorectal cancer and its relationship with the clinicopathological features of colorectal cancer. Methods The expression of EZH2 mRNA in 27 cases of colorectal cancer tissues, paracancerous tissues and normal mucosa epithelial tissues was detected by PCR and its relationship with clinicopathological features was analyzed. Results The expression levels of EZH2 mRNA in colorectal cancer tissues, paracancerous tissues and normal mucosa epithelial tissues were 3.01 ± 1.29,1.20 ± 0.69 and 0.87 ± 0.37, respectively. The expression of EZH2 mRNA in colorectal cancer tissue was the highest (P <0.01) However, there was no significant difference in the expression of paracancer tissues and normal mucosal epithelium (P = 0.403). The expression of EZH2 mRNA in colorectal cancer was correlated with TNM staging (P = 0.002) and histological grade (P = 0.005), but not with age (P = 0.388), sex (P = 0.963), tumor diameter ), Histological type (P = 0.945), tumor location (P = 0.611) and lymph node metastasis (P = 0.449). Conclusions EZH2 mRNA is highly expressed in colorectal cancer tissues and may play a key role in the development and progression of colorectal cancer. It may be used as a reference index to judge the malignancy and progression of colorectal cancer.