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目的探讨异常黑胆质证肿瘤患者体内脂肪酸的代谢规律,通过评价脂肪酸的含量,认知异常黑胆质证肿瘤患者体内脂肪酸代谢特点,寻找异常黑胆质证肿瘤患者体内的差异性代谢物。方法按维医理论分型的诊断标准,选择异常黑胆质证患者123例,非异常黑胆质证患者111例,健康者97例,采用气相色谱(GC)技术对血浆中10种脂肪酸的含量进行测定,并采用t检验和正交偏最小二乘法判别分析(OPLS-DA)对数据进行统计学分析。结果检测发现,OPLS-DA空间分布图中异常黑胆质证肿瘤患者与健康者分类趋势明显;异常黑胆质证肿瘤患者与健康者比较,肉豆蔻酸、棕榈酸、棕油酸、硬脂酸、EPA、花生酸等6种脂肪酸含量差异有统计学意义(P<0.05);异常黑胆质证肿瘤患者与非异常黑胆质肿瘤患者比较;脂肪酸中花生酸的含量差异有统计学意义(P<0.05)。结论 GC技术结合OPLS-DA空间分析模式识别的代谢组学方法具有研究复杂条件下机体病理生理变化的优势,为异常黑胆质证肿瘤患者血浆中脂肪酸成分及含量测定提供了依据,可为预测维医不同证候发生、发展的机制提供理论支持。
Objective To investigate the metabolism of fatty acids in patients with abnormal savda syndrome. To evaluate the fatty acid metabolism in patients with abnormal savda syndrome and to find the differential metabolites in patients with abnormal savda syndrome. Methods According to the diagnostic criteria of the theory of traditional Chinese medicine, 123 patients with abnormal savda syndrome, 111 non-abnormal savda syndrome patients and 97 healthy controls were selected. The contents of 10 fatty acids in plasma were determined by gas chromatography (GC) The data were statistically analyzed by t-test and Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA). Results The results showed that there was a clear trend in the distribution of OPLS-DA spatial distribution in patients with abnormal savda syndrome and healthy subjects. Compared with healthy controls, patients with abnormal savda syndrome showed that myristic acid, palmitic acid, palmitoleic acid, stearic acid, EPA and arachidic acid had statistical significance (P <0.05). There were significant differences in the content of arachidic acid in fatty acid between patients with abnormal savda syndrome and non-abnormal savda tumors (P < 0.05). Conclusion The metabolomics method of GC combined with OPLS-DA spatial pattern recognition has the advantage of studying the pathophysiological changes under complicated conditions and provides a basis for the determination of fatty acid composition and content in the plasma of patients with abnormal savda syndrome. Medical different syndromes occur, the mechanism of development to provide theoretical support.