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以籼型两用核不育水稻ＧＤＩＳ和Ｎ１９Ｓ及常规水稻粳舢８９为材料，研究了自然条件下不同光温引起两用稻育性转换时活性氧清除系统的变化。实验结果表明：３月上旬播种在长日高温下发育的两用稻出现败育，各生育期的剑叶、幼穗，完熟期的颖花和花药中ＡｓＡ（抗坏血酸）和ＧＳＨ（谷胱甘肽）含量均低于同期播种的粳籼８９。在７月中旬播种生长发育期遇短日低温的两用稻呈可育态，这时ＡｓＡ、ＧＳＨ含量明显高于粳籼８９。抗氧化酶活性在可育状态的两用稻中其活性也均高于同期播种的粳籼８９。在两种光温条件下发育的两用稻在各生育期的剑叶中ＰＯＤ（过氧化物酶）活性均高于同期播种的粳籼８９，只是短日低温下发育的两用稻与粳籼８９之间ＰＯＤ活性之差更显著。长日高温引起籼型光温敏核不育系水稻的败育与剑叶和幼穗中活性氧清除能力的下降、活性氧产生与清除之间的失衡导致氧代谢的失调有一定的关系。
The changes of active oxygen scavenging system under different light temperature induced by fertility transition of two-purpose rice under natural conditions were studied using GDIS and N19S of indica dual-purpose nuclear sterile rice and conventional rice japonica rice 89 as materials. The results showed that the dual cropping rice developed in long-day and high temperature in early March aborted, and the contents of AsA and GSH in flag leaf, young panicle, Peptide) were lower than the same date sown japonica rice 89. In mid-July, the dual-use rice with short days and low temperatures during sowing and development was fertile, and the content of AsA and GSH was obviously higher than that of japonica × 89. Antioxidant enzyme activities in fertile state of dual purpose rice were also higher than the activity of the same sowing japonica rice 89. The activity of POD (peroxidase) in flag leaves of all rice cultivars grown under two light and temperature conditions was higher than that of japonica rice 89 sown in the same period, only the two-purpose rice and japonica rice developed at short day and low temperature The difference in POD activity between indica-89 was more pronounced. The prolonged high temperature caused the abortion of indica PHTMS rice and the decrease of active oxygen scavenging ability in flag leaf and young panicle. The imbalance between active oxygen production and scavenging resulted in the imbalance of oxygen metabolism.