论文部分内容阅读
ＨＲＰ注入大鼠一侧终纹床核，存活４８小时，再将１％Ｆｏｒｍａｌｉｎ导入胃内造成内脏伤害刺激，存活２小时后，灌注取材．对延髓切片进行ＨＲＰ是色和抗ＦＯＳ（ＡＢＣ法）、抗ＴＨ（ＰＡＰ法）免疫组化反应的三重反应．在光镜下发现“延髓内脏带”内出现七种阳性细胞，包括ＨＲＰ阳性、ＴＨ和ＦＯＳ免疫反应阳性；ＴＨ／ＨＲＰ、ＦＯＳ／ＨＲＰ、ＦＯＳ／ＴＨ等双重阳性以及ＦＯＳ／ＨＲＰ／ＴＨ三重反应阳性细胞．这些阳性细胞主要见于内脏带背内侧部的孤束核，其次是腹外侧区的腹外侧网状核（ＶＬＲ），中间部较少．ＴＨ和ＦＯＳ免疫阳性细胞以及ＴＨ／ＦＯＳ双重阳性细胞为双侧分布，ＨＲＰ／ＦＯＳ、ＨＲＰ／ＴＨ以及ＨＲＰ／ＴＨ／ＦＯＳ阳性细胞也为双侧分布但以注射区同侧为多。
HRP into the rat’s terminal striation bed nucleus, survival 48 hours, and then 1% Formalin into the stomach to cause visceral injury stimulation, survival 2 hours after the perfusion drawn. The medulla oblongata HRP is a triple reaction of color and anti-FOS (ABC method), anti-TH (PAP method) immunohistochemical reaction. There were seven positive cells in the medullary visceral zone, including HRP positive, TH and FOS immunoreactivity; the double positive of TH / HRP, FOS / HRP and FOS / TH and the triple reaction of FOS / HRP / TH Positive cells. These positive cells are mainly found in the nucleus tractus solitarius of the dorsolateral part of the gut, followed by the ventral reticular nucleus (VLR) of the ventral lateral region, with fewer intermediate parts. TH and FOS immunoreactive cells and TH / FOS double positive cells were bilaterally distributed. HRP / FOS, HRP / TH and HRP / TH / FOS positive cells were also bilaterally distributed but more often in the ipsilateral injection zone.