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用 Ｐ Ｃ Ｒ 方法，从人脑ｃ Ｄ Ｎ Ａ 文库中克隆 Ｆ Ｈ Ｉ Ｔ 基因扩增得到５５３ ｂｐ 片段克隆于ｐ Ｇ Ｅ Ｍ Ｔ 载体，测定了其 Ｄ Ｎ Ａ 序列该序列包括 Ｆ Ｈ Ｉ Ｔ ｃ Ｄ Ｎ Ａ 编码区全部４４４ ｂｐ，以及５′端５２ ｂｐ 和３′端５７ ｂｐ 非编码区序列编码区有二处位点发生突变：第９２位密码子中的 Ｃ突变成 Ｇ，是同义突变，不导致氨基酸改变；第１１２位密码子中的 Ｃ也突变成 Ｇ，导致由 Ｔｈｒ变为 Ｓｅｒ到目前为止尚未发现有 Ｆ Ｈ Ｉ Ｔ 基因编码区内部点突变导致氨基酸改变的报导
Using the P CR method, the F H I T gene was cloned from the human brain c D N A library and amplified to obtain a 553 bp fragment and cloned into the p G E M T carrier. Its D N A sequence was determined and the sequence was included. All of the 444 bp coding regions of H I T c D N A and the 52 bp 5’ and 57 bp non-coding region of the 3’ end were mutated at two sites: the C mutation in the 92nd codon. G, a synonymous mutation, does not cause an amino acid change; C in codon 112 also mutates to G, resulting in a change from Thr to Ser. No point mutation has been found in the coding region of the FH I T gene so far. Reports leading to amino acid changes