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目的本研究主要探讨ERCC2和BAG-1基因的多态性与非小细胞肺癌的发病风险、化疗敏感性的相关性。方法采用PCR-RFLP方法对105例非小细胞肺癌患者新鲜血标本中ERCC2/XPDLys751Gln和BAG-1codon 324多态性进行检测,采用SAS软件统计(v.9.1.3e),使用logistic回归分析各基因型与肺癌发病风险和化疗敏感性的相关性。使用比值比(OR)及95%可信区间(CI)表示各基因型与肺癌发病的风险。所有的统计检验均为双侧检验。结果调整了年龄、性别和吸烟后,ERCC2/XPDLys751Gln与肺癌的发病风险无关。携带BAG-1codon 324C/T+T/T基因型者与携带C/C基因型者相比,发病风险降低。携带ERCC2/XPDLys751Gln C/A基因型和A/A基因型者与C/C基因型者相比,与化疗敏感性有关;BAG-1codon 324C/T型与C/C型基因型相比,与化疗敏感性有关。结论ERCC2/XPD、BAG-1基因多态性与非小细胞肺癌发病风险相关,ERCC2/XPD C/A基因型与化疗敏感性具有相关性;BAG-1codon324C/T基因型患者比C/C基因型患者化疗敏感。
The purpose of this study is to investigate the relationship between the polymorphisms of ERCC2 and BAG-1 genes and the risk of non-small cell lung cancer and chemosensitivity. Methods PCR-RFLP was used to detect the polymorphisms of ERCC2 / XPDLys751Gln and BAG-1codon 324 in 105 non-small cell lung cancer patients using SAS software (v.9.1.3e) and logistic regression analysis of each gene Type and lung cancer risk and chemotherapy sensitivity correlation. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to indicate the risk of each genotype and lung cancer. All statistical tests are two-sided test. Results After adjustment for age, sex and smoking, ERCC2 / XPDLys751Gln was not associated with the risk of lung cancer. Patients with the BAG-1 codon 324C / T + T / T genotype had a lower risk of developing disease than those with the C / C genotype. Compared with C / C genotypes, those with ERCC2 / XPDLys751Gln C / A genotype and A / A genotype were associated with chemosensitivity. Compared with C / C genotype, BAG-1codon 324C / T genotype Chemosensitivity related. Conclusion The polymorphisms of ERCC2 / XPD and BAG-1 are associated with the risk of non-small cell lung cancer. The genotype of ERCC2 / XPD C / A is correlated with chemosensitivity. Compared with C / C genotypes, BCC-1codon324C / Chemotherapy-sensitive patients.