论文部分内容阅读
Neuronal connections can be detected by neuronal network discharges in hippocampal neurons cultured on multi-electrodes. However, the multi-electrode-array (MEA) has not been widely used in hippocampal slice culture studies focused on epilepsy. The present study induced spontaneous synchronous epileptiform activity using low Mg2+ artificial cerebrospinal fluid on acute hippocampal slices to record hippocampal discharges with MEA. Results showed that burst duration and average number of spikes in a burst were significantly greater in the CA3 compared with dentate gyrus and CA1 areas. In Schaffer cut-off group, CA1 area discharges disappeared, but synchronous discharges remained in the CA3 area. Moreover, synchronous discharge frequency in the Schaffer cut-off group was similar to control. However, burst duration and average number of spikes in a burst were significantly decreased compared with control (P < 0.05). Results demonstrated that highest neuronal excitability occurred in the CA3 area, and synchronous discharges induced by low Mg2+ originated from the CA3 region.
However, the multi-electrode-array (MEA) has not been widely used in hippocampal slice culture studies focused on epilepsy. The present study induced spontaneous synchronous epileptiform activity of low Mg2 + artificial cerebrospinal fluid on acute hippocampal slices to record hippocampal discharges with MEA. In Schaffer cut-off group, CA1 area discharges disappeared, but synchronous discharges remained in the CA3 area. However, the burst duration and average number of spikes in a burst were significantly decreased compared with control (P <0.05). Results said that highest neuronal excitability occurred in the CA 3 area, and synchronous discharges induced by low Mg2 + originated from the CA3 region.