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利用反转录 Ｐ Ｃ Ｒ 方法，对甜菜坏死黄脉病毒（ Ｂ Ｎ Ｙ Ｖ Ｖ）５ 个中国分离物的 Ｒ Ｎ Ａ５进行了检测，结果表明新疆分离物、黑龙江分离物和内蒙古乌拉特前旗分离物不含有 Ｒ Ｎ Ａ５ ，只有包头分离物和呼和浩特分离物有 Ｒ Ｎ Ａ５ 。序列分析结果，包头分离物和呼和浩特分离物 Ｒ Ｎ Ａ５ 基因组分别为１３３８ ｎｔ 和１３５８ ｎｔ，均只包含１ 个开放阅读框架，编码产生２６ ｋ Ｄ 蛋白。与法国 Ｆ７２ 分离物和日本 Ｄ５ 分离物相比， 各分离物间核苷酸序列同源性为９３７ ％ ～９８５ ％ ，由此推导的氨基酸序列同源性为９１８ ％ ～９８２ ％ 。
The reverse transcriptase-PCR method was used to detect the R N A5 of five Chinese isolate of beet necrotic yellow vein virus (B N Y V V). The results showed that Xinjiang isolates, Heilongjiang isolates and Inner Mongolia Wulate The flag isolates do not contain R N A5, only the Baotou isolates and Hohhot isolates have R N A5. The results of sequence analysis showed that the R N A5 genome of Baotou isolates and Huhhot isolates were 1338 nt and 1358 nt, respectively, which contained only one open reading frame and encoded a 26 kD protein. Compared with the French F72 isolate and the Japanese D5 isolate, the nucleotide sequence homology among the isolates was 93.7% -98.5%, and the deduced amino acid sequence identity was 91.8% 98 2%.