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本文以茄子抗青枯病自交系和感病自交系为试材,在接种青枯病菌后,对调控抗性反应的不同信号传导基因进行表达特性分析。结果表明,在9个基因中,EDS1、PAD4、NPR1、SGT1和WRYK70等5个基因的表达均随着接种诱导时间延长而增加,而且在抗病自交系‘E-31’中的表达量要高于感病自交系‘E-32’;而JAR1、NDR1、EIN2和RAR1等基因的表达随着接种诱导时间的增加,表达水平变化不大。初步推断EDS1、PAD4、NPR1、SGT1和WRYK70可能与调节茄子抗青枯病抗性反应有关。同时茄子在接种后活性氧的含量均增加,但是感病植株的含量高于抗病植株。
In this study, the inbred lines and susceptible inbred lines of eggplant resistant to bacterial wilt were used as test materials. After inoculation with bacterial wilt, the expression characteristics of different signal transduction genes regulating resistance were analyzed. The results showed that the expression of EDS1, PAD4, NPR1, SGT1 and WRYK70 among 9 genes increased with the induction of inoculation time, and the expression level in E-31 ’ Which was higher than susceptible inbred lines ’E-32’. However, the expression of JAR1, NDR1, EIN2 and RAR1 genes did not change much with inoculation time. Preliminary inference EDS1, PAD4, NPR1, SGT1 and WRYK70 may be related to the regulation of eggplant resistance to bacterial wilt. At the same time, the content of reactive oxygen species of eggplant increased after inoculation, but the content of susceptible plants was higher than that of disease-resistant plants.