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目的了解绵阳市食品中单增李斯特菌的污染及耐药状况,为预防控制该菌引起的食源性疾病提供科学依据。方法菌株分离采用GB 4789.30-2010方法,菌株鉴定采用API LISTERIA生化鉴定条,菌株药敏试验采用K-B法。结果 114份食品中共检出11株单增李斯特菌,总检出率为9.7%,生食水产品、生禽肉、生畜肉和豆制品4种食品受到该菌的污染,检出率分别为33.3%、26.7%、6.7%、6.7%。分离的11株菌株氨苄西林、阿莫西林/克拉维酸、复方新诺明、磺胺嘧啶、庆大霉素、阿米卡星、环丙沙星、头孢噻吩、氯霉素9种抗生素全部敏感;对头孢曲松和头孢噻吩没有敏感株。结论绵阳市的部分食品受到单增李斯特菌不同程度的污染,生食水产品受该菌污染最严重,其次是生禽肉,有发生食源性李斯特菌病的潜在风险。单增李斯特菌的耐药性较低,对多种抗生素敏感。应高度重视和加强食源性单增李斯特菌的污染状况及耐药性监测,以保证食品安全和人类健康。
Objective To understand the contamination and drug resistance status of Listeria monocytogenes in food in Mianyang City and provide a scientific basis for the prevention and control of foodborne diseases caused by this bacteria. Methods Isolation of strains using GB 4789.30-2010 method, strains identified using API LISTERIA biochemical identification of strains susceptibility test using K-B method. Results 11 strains of Listeria monocytogenes were detected in 114 foodstuffs with a total detection rate of 9.7%. Four kinds of foodstuffs including raw aquatic products, raw poultry, raw meat and soy products were contaminated by this strain. The detection rates were 33.3%, 26.7%, 6.7%, 6.7%. Ampicillin, amoxicillin / clavulanic acid, cotrimoxazole, sulfadiazine, gentamycin, amikacin, ciprofloxacin, cefalotin and chloramphenicol were all sensitive to the 11 isolates ; No sensitivity to ceftriaxone and cefalotin. Conclusion Some foods in Mianyang City were contaminated by Listeria monocytogenes to some extent. Raw food aquatic products were the most polluted by the bacteria, followed by raw poultry meat, with the potential risk of foodborne listeriosis. Listeria monocytogenes is less resistant and susceptible to multiple antibiotics. The status of foodborne Listeria monocytogenes contamination and drug resistance should be highly valued and strengthened to ensure food safety and human health.