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目的了解病案染菌情况,评价臭氧消毒灭菌效果,探讨防止接触病案的人员免受相关感染的方法。方法分别对灭菌前后半小时的病案取样,进行细菌培养计数及鉴定。结果消毒灭菌前平均细菌菌落数5.3CFU/ml,消毒后灭菌平均细菌菌落数0.4CFU/ml。灭菌前细菌除表皮葡萄球菌、芽胞杆菌、棒状杆菌属、真菌(霉菌)等环境中常见菌之外,金黄色葡萄球菌、铜绿假单胞菌、鲍曼不动杆菌、大肠埃希菌等院内感染最常见病原菌也大量存在。芽胞杆菌、真菌消毒效果应该相对较差。结论病案在形成、使用过程中,受到各种细菌污染,因此要重视病案灭菌,减少使用病案人员免受细菌感染的机会。
Objective To understand the situation of disease contamination, to evaluate the effectiveness of ozone disinfection and sterilization, and to explore ways to prevent people exposed to the disease from being infected. Methods Samples were taken for half an hour before and after sterilization, and bacterial cultures were counted and identified. Results The average number of bacterial colonies before sterilization and sterilization was 5.3 CFU/ml, and the average number of bacterial colonies after sterilization was 0.4 CFU/ml. Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, Escherichia coli, etc. other than common bacteria in the environment such as Staphylococcus epidermidis, Bacillus, Corynebacterium, fungi (mold) before sterilization Nosocomial infections are the most common pathogens. Bacillus and fungal disinfection should be relatively poor. Conclusion During the process of formation and use, the medical record is contaminated by various bacteria. Therefore, it is necessary to attach importance to the sterilization of the case and reduce the chance of using the patient to prevent bacterial infection.