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虽然促皮质释放因子(CRF)是在下丘脑发现的第一种激素,但它的化学结构仍不清楚。有人报导从羊的丘脑下部分离得一种41肽,该肽在小鼠体内或体外都具有CRF活性。本文报导,用人工合成的羊CRF能刺激正常人垂体前叶分泌ACTH。方法:选正常男性受试者6人(年龄29～35岁)。禁食一夜后在早晨插入静脉前臂插管,45分钟后抽取基础血液标本1份,间隔15分钟后再取1份。然后分别向受试者注入含100μg合成CRF的酸性盐溶液1ml或1ml生理盐溶液,起初每间隔15分钟抽一次血标本,共4次;然后每隔30分钟抽一次,共2次。在至少一周后,用双盲法检查每一例受试者血液,作为各自的对照。试验期间,受试者一直卧床。每
Although corticotropin-releasing factor (CRF) is the first hormone found in the hypothalamus, its chemical structure remains unclear. It has been reported that a 41 peptide is isolated from the hypothalamus of sheep, which peptide has CRF activity in vivo or in vitro in mice. This article reports that synthetic sheep CRF can stimulate normal human anterior pituitary secretion of ACTH. Methods: Normal male subjects were 6 (29-35 years old). One night after fasting in the morning after the introduction of intravenous forearm intubation, 45 minutes after the extraction of the basis of a blood specimen, an interval of 15 minutes and then take a copy. Subjects were then injected with 1 ml or 1 ml of saline solution containing 100 μg of synthetic CRF in acid salt solution. Initially blood samples were taken at 15-minute intervals for a total of 4 times, and then every 30 minutes for 2 times. After at least one week, each subject blood was examined by double-blind as the respective control. During the test, the subject remained in bed. each