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目的：了解GM－CSF对肺癌细胞的杀伤作用以及时其凋亡和对AM抗肿瘤功能的影响。方法：MTT观察GM－CSf对人肺腺癌细胞株（A549）细胞的杀伤作用以及对肺癌患者AM杀伤A549细胞的影响。透射电镜、凝胶电泳及片段裂解率观察经CM－CSF作用的A549细胞凋亡情况．镀钢镉还原法、放免法、比色法分别检测AM培养上清中NO、TNFα、SOD活性，并用RT．PCR检测AMiNOSmRNA表达。结果：GM－CSF对A549细胞有一定杀伤作用，并呈浓度依赖性。A549细胞核扭曲断裂，凋亡小体形成，DNA凝胶电泳可见梯状条带。AM经GM－CSF刺激后对A549细胞的杀伤率由14.35%增加至4352%（P＜0．001），其培养上清波中NO、TNFα的水平显著升高，AMiNOSmRNA表达明显增强。结论：GM－CSF可诱导A549细胞凋亡，刺激肺癌患者AM抗肿瘤功能增强。
Objective: To understand the killing effect of GM-CSF on lung cancer cells and its effect on the antitumor activity of AM. METHODS: MTT assay was used to observe the killing effect of GM-CSf on human lung adenocarcinoma cell line (A549) and the effect of AM killing on A549 cells. Transmission electron microscopy, gel electrophoresis and fragment lysis rate were used to observe the apoptosis of A549 cells treated with CM-CSF. The steel plating cadmium reduction method, radioimmunoassay, and colorimetry were used to detect the activity of NO, TNFα, and SOD in AM culture supernatant, respectively, and RT was used. AmiNOS mRNA expression was detected by PCR. Results: GM-CSF had a certain killing effect on A549 cells in a concentration-dependent manner. The nuclei of A549 cells distorted and disintegrated, apoptotic bodies formed, and ladder bands appeared on DNA gel electrophoresis. The killing rate of AM in A549 cells stimulated by GM-CSF increased from 14.35% to 4352% (P<0.001). The levels of NO and TNFα in the culture supernatant wave were significantly increased, and the expression of AMiNOS mRNA was significantly increased. Conclusion: GM-CSF can induce apoptosis of A549 cells and stimulate the enhancement of AM anti-tumor function in lung cancer patients.