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目的 探讨大鼠实验性脑出血后脑组织中即刻早期基因c fos的表达和局部脑血流的变化。方法 采用Nath改良法建立大鼠脑出血模型 ;免疫组化法及RT PCR法测定其脑组织中fos蛋白和c fosmRNA的表达 ;氢清除法测定其局部脑血流。结果 大鼠血肿周围区 (基底节 )在脑出血后 1小时即出现fos蛋白的表达 ,至 3小时达高峰 ;c fosmRNA于出血后 1小时达表达高峰 ,至 3小时后仍有较高水平的表达 ;出血后 1小时全脑的血流量均下降 ,4小时恢复至对照组水平 ,并维持至出血后 2 4小时 ,随后的 2 4小时内再次出现脑血流下降。结论 大鼠脑出血后 ,血肿周围区和双侧皮质区的脑组织中存在着c fos基因的快速而长久的诱导表达。局部脑血流的下降相对短暂 ,且脑血流的下降在时程上与c fos基因的表达不相一致
Objective To investigate the expression of immediate early gene c fos and changes of regional cerebral blood flow in rat brain after experimental intracerebral hemorrhage. Methods The rat model of intracerebral hemorrhage was established by Nath method. The expression of fos protein and c fos mRNA in brain tissue were determined by immunohistochemistry and RT PCR. The regional cerebral blood flow was measured by hydrogen scavenging assay. Results The expression of fos protein in the area around the hematoma (basal ganglia) appeared at 1 hour after intracerebral hemorrhage and peaked at 3 hours. The peak of c fos mRNA reached its peak at 1 hour after hemorrhage and remained high at 3 hours 1 hour after hemorrhagic blood flow decreased in the whole brain, returned to the level of 4 hours after the control group and maintained until 24 hours after the hemorrhage, and then within 24 hours after the decline of cerebral blood flow again. Conclusion After intracerebral hemorrhage in rats, there is a fast and long-term induction of c fos gene in the brain tissue around the hematoma and bilateral cortex. The decline in local cerebral blood flow is relatively short, and the decline in cerebral blood flow does not coincide with the expression of the c fos gene on a time course