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[目的]制备一种简便、实用的急性大鼠脊髓损伤建模装置。[方法]用木板、新泡沫板、吸管、手术缝线及金属插销杆等制作新型大鼠脊髓打击器。取36只成年雌性SD大鼠,体质量为220~250 g,随机等分为假手术组(Sham组)、轻度打击组(SCI1组)和重度打击组(SCI2组),利用自制大鼠脊髓打击器建立大鼠脊髓损伤模型,SCI1组采用10 g×25 mm打击规格进行垂直打击损伤,SCI2组采用10 g×50 mm打击规格进行垂直打击损伤。各组大鼠于术前1 d,术后1、3、7、14、21和28 d进行BBB运动学评分,观察大鼠后肢运动功能情况,术后28 d对各组大鼠受损脊髓组织进行HE染色、神经元核抗原(Neu N)免疫组化检测。[结果]Sham组各观察时间点的BBB评分均为21分,各SCI组术后1 d时BBB评分为0分,之后BBB评分结果随时间延长呈逐步恢复趋势,从3 d开始,SCI1组的BBB评分高于SCI2组(P<0.05)。各组大鼠后肢左右侧活动情况无明显差异,实验过程中无大鼠死亡。术后28 d HE染色、Neu N免疫组化结果显示SCI2组脊髓受损程度明显重于SCI1组(P<0.05)。[结论]该新型大鼠脊髓打击器制作简便、可行性高,建立的SCI模型具有稳定性高、可重复性强、损伤程度可调节等特点,适合相关科研人员使用。
[Objective] To prepare a simple and practical model of acute spinal cord injury in rats. [Method] A new rat spinal cord striker was made with wood board, new foam board, pipette, surgical suture and metal latch rod. Thirty-six adult female Sprague-Dawley rats weighing 220-250 g were randomly divided into sham operation group (SCI1) and sham operation group (SCI2) Spinal cord injury model was established in rats. SCI1 group was impacted vertically with 10 g × 25 mm and SCI2 group with 10 g × 50 mm hitting scale. The BBB kinematic scores of rats in each group were observed on the first day before operation and on the 1st, 3rd, 7th, 14th, 21st and 28th day after operation. The motor function of the hindlimbs was observed. The injured spinal cord Tissues were subjected to HE staining and immunohistochemical detection of neuronal nuclear antigen (Neu N). [Results] The BBB score of Sham group at each observation time point was 21 points, and the BBB score at 0th day after operation in each SCI group was 0. BBB score tended to recover gradually with time. From the 3th day, the SCI1 group BBB score was higher than SCI2 group (P <0.05). There was no significant difference in left and right hindlimb activities of rats in each group, and no rats died during the experiment. The results of Neu N immunohistochemistry showed that the degree of spinal cord injury in SCI2 group was significantly heavier than that in SCI1 group (P <0.05). [Conclusion] The new rat spinal cord striker is easy to make and feasible. The established SCI model has the characteristics of high stability, strong repeatability and adjustable degree of injury, which is suitable for the related scientific researchers.