论文部分内容阅读
该实验研究了野菊光周期途径关键基因CO的表达模式, 采用RT-PCR法克隆得到野菊CO基因的CDS序列, 其开放阅读框长1 380 bp, 编码459个氨基酸.生物信息学分析表明, 野菊CO与甘菊、黄花蒿同源性较高, 表现为CO在同科内较为保守.预测得到该蛋白的相对分子质量约为52. 04 k Da, 理论等电点为4. 81, α螺旋占17. 65％, 随机卷曲占76. 69％, 延伸链占5. 66％, 无β折叠, 无信号肽.实验结果显示短日照处理可以提高野菊CO基因的表达量并诱导其开花; qRT-PCR结果显示CO基因在野菊不同组织及不同处理时期相对表达量差异显著.该文通过研究短日照处理对野菊光周期途径开花关键基因CO表达量的影响, 为光周期调控野菊花期、采收期提供研究基础.“,”This experiment studied the expression pattern of key gene CO in the photoperiod of Chrysanthemum indicum. The CDS sequence of the Ch. indicum CO gene was cloned by RT-PCR. The open reading frame was 1 380 bp in length and encoded 459 amino acids. The bioinformatics analysis results showed that the Ch. indicum CO had higher homology with Ch. lavandulifolium and Artemisia annua, and the CO was more conservative in the same family. The molecular weight of the predicted protein encode by CO is 52. 04 k Da, the p I is 4. 81, the α-helix structure accounted for 17. 65％, the random coil accounted for 76. 69％, the extension chain accounted for 5. 66％, there are no β-fold and signal peptide. The experimental results showed that short-day treatment could increase the expression level of CO gene in Ch. indicum and induce its flowering. The results of qRT-PCR showed that the relative expression of CO gene in different tissues and different treatment periods of Ch. indicum was significantly different. In this paper, we studied the effect of short-day treatment on the expression of key genes in the flowering cycle of Ch. indicum, providing a basis for photoperiod regulation and harvesting period of Ch.indicum.