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应用补肾益精中药(固真方)喂饲雄性老年大鼠,观察用药后胸腺超微结构的变化。动物分三组:青年组,16月令;老年组,22月令;中药组,22月令,从18月令开始每日饲喂中药持续四个月。三组动物杀死后迅速取出胸腺,以常规电镜制样技术进行固定、脱水、包埋、超薄切片、染色,电镜观察。老年胸腺与青年胸腺比较,胸腺组织内上皮性网状细胞与淋巴细胞皆显示超微结构显著变化。细胞核周间隙扩张,细胞质内细胞器变性,线粒体肿胀,嵴断裂或消失,基质电子密度降低,粗面内质网减少並有脱颗粒现象,内质网池扩张,次级溶酶体数量增加[图1]。而中药组(固真方)老年动物胸腺组织上皮性网状细胞与
The rats were fed with Bushen Yijing Pharmacopoeia (Guizhenfang) to observe the changes of the thymus ultrastructure in the aged rats. The animals were divided into three groups: the youth group, the 16-month order; the elderly group, the 22-month order; the Chinese herbal medicine group, the 22-month order, which started daily feeding of Chinese herbs for four months from the 18th month. After three groups of animals were killed, the thymus was quickly removed, fixed, dehydrated, embedded, ultrathin sectioned, stained and electron microscopically observed by conventional electron microscopy. Compared with the young thymus, the ultrastructural changes of the epithelial reticulocytes and lymphocytes in the thymus tissues were observed. Cytoplasmic peritumoral dilation, cytoplasmic organelles degeneration, mitochondria swelling, crista fracture or disappearance of matrix electron density decreased rough endoplasmic reticulum reduction and degranulation, endoplasmic reticulum pool expansion, the number of secondary lysosomes increased [Figure 1]. The traditional Chinese medicine group (Guzhen Fang) thymus in elderly animal epithelial reticular cells and