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目的探讨ERK1/2丝裂素活化蛋白激酶信号转导途径在DHA抑制3T3-L1前脂肪细胞增殖中的作用。方法四唑盐比色法(MTT法)检测DHA处理24h对体外培养的3T3-L1前脂肪细胞活力的影响,流式细胞术检测细胞周期,蛋白免疫印迹法检测ERK1/2、p-ERK1/2、p21水平。结果 MTT结果显示DHA干预24h可剂量依赖性降低3T3-L1前脂肪细胞的活力,抑制增殖,IC50为100μmol/L;流式细胞术结果表明,DHA可将3T3-L1前脂肪细胞阻滞在G2/M期;蛋白免疫印迹发现,DHA可明显升高3T3-L1前脂肪细胞ERK1/2磷酸化水平、增强p21蛋白表达。结论 DHA可能通过活化ERK1/2通路诱导G2/M期阻滞,进而抑制3T3-L1前脂肪细胞增殖。
Objective To investigate the role of mitogen-activated protein kinase (ERK1 / 2) signaling pathway in the inhibition of 3T3-L1 preadipocyte proliferation by DHA. Methods MTT method was used to detect the effect of DHA treatment on the viability of 3T3-L1 preadipocytes cultured in vitro. The cell cycle was detected by flow cytometry. The expressions of ERK1 / 2 and p-ERK1 / 2, p21 level. Results MTT results showed that DHA could inhibit the proliferation of 3T3-L1 preadipocytes in a dose-dependent manner for 24 h with IC50 of 100 μmol / L in a dose-dependent manner. Flow cytometry showed that DHA blocked 3T3-L1 preadipocytes in G2 / M phase. Western blotting showed that DHA significantly increased ERK1 / 2 phosphorylation and increased p21 protein expression in 3T3-L1 preadipocytes. Conclusion DHA may induce G2 / M arrest through activation of ERK1 / 2 pathway, thereby inhibiting 3T3-L1 preadipocyte proliferation.