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为了解决用常现病理切片技术难以发现淋巴结微转移这一缺憾，作者在1992．4～1995．7期间，对68例食管癌根治术共399个常规病理检查认为没有肿瘤转移的淋巴结标本用免疫组化技术进行了研究。这些标本用抗上皮细胞的单克隆抗体Ber－EP。处理，以恒冷切片机在淋巴结的不同平面切
In order to solve the shortcomings of difficult to detect lymph node micrometastases using routine pathological biopsy, the authors performed immunohistochemistry on lymph node specimens without metastasis in 399 routine pathological examinations of 68 cases of esophageal cancer during the period from 1992.4 to 1995.7. The grouping technique was studied. These specimens were used anti-epithelial monoclonal antibody Ber-EP. Treated with cryostat to slice in different planes of lymph nodes