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目的:探讨转录因子激活增强子结合蛋白4(TFAP4)在胃癌中对LINC01235表达的调控作用。方法:通过生物信息学分析，获取TFAP4可在胃癌中调控的LINC01235。采用实时荧光定量聚合酶链反应(Real-time PCR)检测在胃癌细胞中敲低TFAP4后LINC01235的表达变化。应用染色质免疫共沉淀(ChIP)法验证TFAP4可以结合LINC01235的启动子区域调控其转录。采取双荧光素酶报告实验计算相对荧光活性验证启动子区域的单核苷酸多态性(SNP) rs34667091可以促进转录。对独立样本采用n t检验。n 结果:肿瘤基因组图谱(TCGA)数据库中胃癌RNA-seq数据进行分析，可见LINC01235的高表达与不良预后明显相关(n P<0.01)。JASPAR网站预测TFAP4可以调控LINC01235的表达。在胃癌细胞系中敲低TFAP4的表达后，LINC01235的表达水平随之降低。ChIP实验结果显示，与阴性对照组IgG比较，TFAP4与LINC01235的启动子区域结合富集百分比较高(0.000 299±2.129e-005比0.001 249±1.985e-005，n t＝32.640，n P<0.01)，差异有统计学意义。双荧光素酶报告实验结果显示，与正常对照组比较，LINC01235启动子区域的SNPs缺失后，TFAP4与启动子区域结合的相对荧光活性降低(1.000±0.207比0.341±0.031，n t＝3.151，n P<0.05；1.000±0.156比0.157±0.042，n t＝5.221，n P<0.05)，差异均有统计学意义。n 结论:LINC01235的表达水平在胃癌中和不良预后呈正相关，TFAP4可以调控LINC01235的转录，LINC01235的启动子区域的SNP rs34667091可作为增强子。“,”Objective:To investigate the regulatory effect of transcription factor activating enhancer-binding protein 4 (TFAP4) on the expression of LINC01235 in gastric cancer.Methods:Through bioinformatics analysis, LINC01235, which can be regulated by TFAP4 in gastric cancer, was obtained. Thr real-time quantitative polymerase chain reaction (Real-time PCR) was used to detect the expression changes of LINC01235 after knocking down TFAP4 in gastric cancer cells. Chromatin immunoprecipitation (ChIP) method was used to verify that TFAP4 can bind to the promoter region of LINC01235 to regulate its transcription. The dual luciferase reporter experiment was used to calculate the relative fluorescence activity to verify that the single nucleotide polymorphism (SNP) rs34667091 in the promoter region can promote transcription.Results:Analysis of the RNA-seq data of gastric cancer in the TCGA database showed that the high expression of LINC01235 was significantly related to the poor prognosis (n P<0.01). JASPAR website predicts that TFAP4 can regulate the expression of LINC01235. After knocking down the expression of TFAP4 in gastric cancer cell lines, the expression level of LINC01235 decreased. The results of the ChIP experiment showed that the binding enrichment percentage of TFAP4 and the promoter region of LINC01235 was higher (0.000 299±2.129e-005) than in the negative control IgG (0.001 249±1.985e-005,n t=32.640, n P<0.01). The results of the dual luciferase report experiment showed that as compared with the normal control group, after the SNPs in the promoter region of LINC01235 were deleted, the relative fluorescence activity of TFAP4 bound to the promoter region significantly decreased (1.000±0.207 vs. 0.341±0.031,n t=3.151, n P<0.05; 1.000±0.156 vs. 0.157±0.042,n t=5.221, n P<0.05).n Conclusion:The expression level of LINC01235 is positively correlated with poor prognosis in gastric cancer. TFAP4 can regulate the transcription of LINC01235, and the SNP rs34667091 in the promoter region of LINC01235 can act as an enhancer.