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本文对比研究了19种抗菌药物对40株绿脓杆菌体外抗菌活性。抗菌药物包括7种半合成青霉素类、6种第3代头孢菌素类、5种氨基糖苷类抗生素及1种喹诺酮类抗菌药物。应用琼脂平板稀释法测定MIC范围、MIC_(50)及MIC_(90)。实验结果证明,Ceftazidime是19种抗菌药物中抗绿脓杆菌活性最强者(MIC0.5～16μg/ml)。酰脲类青霉素如阿洛西林、呋苄青霉素和哌拉西林抗绿脓杆菌活性也较高,在浓度为8μg/ml时,分别可抑制92.5％、90％和85％菌株。Cefsulodin和头孢哌酮也具有较高的抗绿脓杆菌活性,在浓度为8μg/ml时,分别可抑制92.5％和95％菌株。上述抗菌药物对绿脓杆菌的体外抗菌活性可与氨基糖苷类抗生素媲美。 作者对比了我院分离与北京市其他医院分离的绿脓杆菌对19种抗菌药物的敏感性,其中5种青霉素类及3种头孢菌素类对我院分离菌株的MIC明显高于外院,此结果与用药特点不同有关。同时提示我院分离菌株对β-内酰胺类抗生素敏感性下降,应引起临床医生注意。 同时应用棋盘法测定了阿洛西林和苯唑西林、哌拉西林与氟嗪酸对4株耐羧苄青霉素绿脓杆菌的联合药敏。结果表明,两组组合均显示了协同或部分协同效应。
In this paper, the comparative study of 19 kinds of antibacterial drugs against 40 Pseudomonas aeruginosa in vitro antibacterial activity. Antibacterials include seven semi-synthetic penicillins, six third-generation cephalosporins, five aminoglycoside antibiotics and a quinolone antibacterial. The MIC range, MIC_ (50) and MIC_ (90) were determined by agar plate dilution method. The experimental results show that, Ceftazidime is the antimicrobial activity of 19 anti-Pseudomonas aeruginosa most active (MIC 0.5 ~ 16μg / ml). Uremic penicillins such as azlocillin, felocicline and piperacillin were also more active against Pseudomonas aeruginosa and inhibited 92.5%, 90% and 85% of the strains, respectively, at a concentration of 8 μg / ml. Cefsulodin and cefoperazone also had higher anti-Pseudomonas aeruginosa activity, which inhibited 92.5% and 95% of the strains, respectively, at a concentration of 8 μg / ml. The above antibacterials against Pseudomonas aeruginosa in vitro antibacterial activity with aminoglycoside antibiotics comparable. The authors compared the susceptibility of Pseudomonas aeruginosa to 19 antimicrobial agents isolated in our hospital from other hospitals in Beijing. MICs of 5 penicillins and 3 cephalosporins isolated from our hospital were significantly higher than those seen outside the hospital, The results and the different characteristics of the drug. At the same time prompted the separation of strains in our hospital susceptibility to β-lactam antibiotics, should be caused by clinicians attention. At the same time, the combined sensitivity of azlocillin, oxacillin, piperacillin and flurazinic acid against 4 strains of carbenicillin-resistant Pseudomonas aeruginosa was determined by the checkerboard method. The results showed that both groups showed synergistic or partial synergistic effects.