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目的:通过hulth造模方式建立兔KOA早中期模型,并通过丹参注射液干预观察对兔KOA早中期软骨形态学和软骨细胞中IGF-1,TGF-β的影响。方法:将40只雄性新西兰大白兔随机分为四组,每组10只:正常组、模型组、对照组、丹参组。除正常组外其余各组均按hulth方式造模建立兔KOA早中期模型。对照组与丹参组分别于造模后第2周和第3周开始进行关节腔注射治疗,对照组注射0.3mL透明玻璃脂酸钠,每周1次,共5次;丹参组注射丹参注射液0.3mL,每周1次,共5次;治疗结束后分别于造模后第6周和第8周处死动物,对关节软骨进行大体观察和组织形态学观察,采集关节软骨标本运用免疫组织化学对软骨中IGF-1、FGF-β的含量进行检测。结果:与正常组比较其余各组兔KOA早中期关节软骨发生明显退行性改变及滑膜增生改变,丹参组及对照组经治疗后,关节软骨中IGF-1、TGF-β的含量明显增高,与模型组比较有显著性差异(P<0.05),经肉眼及光镜下观察治疗组关节软骨病理改变明显轻于模型组(P<0.05)。结论:丹参注射液对兔KOA早中期关节软骨退变有防治作用,其可能是通过提高IGF-1、TGF-β的含量,从而促进软骨基质的合成及抑制其分解来实现。
OBJECTIVE: To establish an early and mid-term rabbit model of KOA by hulth modelling, and to observe the effects of Danshen injection on cartilage morphology and cartilage IGF-1, TGF-β in rabbit KOA. METHODS: Forty male New Zealand white rabbits were randomly divided into four groups of 10 in each group: normal group, model group, control group, and Salvia miltiorrhiza group. In addition to the normal group, all other groups were modeled by hulth to establish the early and mid-term model of rabbit KOA. The control group and the Salvia miltiorrhiza group were treated with intra-articular injection at the 2nd and 3rd week after the model was established respectively. The control group was injected with 0.3 mL of sodium hyaluronate, once a week for 5 times; the Danshen group was injected with the Danshen injection. 0.3mL once a week for 5 times; After the end of treatment, the animals were sacrificed at the 6th and 8th weeks after modeling, gross observation and histomorphological observation of articular cartilage were performed, and articular cartilage specimens were collected for immunohistochemistry. The contents of IGF-1 and FGF-β in cartilage were examined. RESULTS: Compared with the normal group, articular cartilage in the remaining rabbits showed significant degenerative change and synovial hyperplasia in the early and middle stages. After treatment, the content of IGF-1 and TGF-beta in the articular cartilage was significantly higher in the Dan Shen group and the control group. There was a significant difference between the model group and the model group (P<0.05). The pathological changes of the articular cartilage in the treatment group were significantly lighter than those in the model group after gross and light microscopy (P<0.05). Conclusion: Salvia miltiorrhiza injection can prevent and treat articular cartilage degeneration of rabbit KOA in the early and middle stage. It may be achieved by increasing the content of IGF-1 and TGF-β, thus promoting the synthesis of cartilage matrix and inhibiting its decomposition.