论文部分内容阅读
生物降解与土著微生物群落结构、功能及其变化密切相关.目前,对于东北冻土土壤中的适冷降解菌了解不足.新建成的中俄输油管道穿越中国东北的多年冻土区,为相关研究提供了契机.实验利用454高通量测序分析了加格达奇冻土活动层土壤在受控原油污染前后的微生物群落结构.结果显示:污染后的Proteobacteria和Firmicutes相对丰度显著升高,优势类群包括Alicyclobacillus、Sphingomonas、Nevskia以及Bacillus.群落以芳烃降解菌或者耐受油污环境的细菌为主.这种变化与原油(尤其是芳烃)组分的生态毒害作用有关.较高浓度的原油污染下,群落中可耐受油污环境的细菌丰度相对更高.
Biodegradation is closely related to the structure, function and changes of indigenous microbial communities, and nowadays, there is a lack of understanding of suitable cold-degrading bacteria in soil in Northeast frozen soil.The newly-built Sino-Russia oil pipeline crosses permafrost regions in Northeast China, Provided an opportunity to analyze the microbial community structure of the soils in the frozen Tarim before and after contaminated by controlled crude oil using 454 high-throughput sequencing.The results showed that the relative abundance of Proteobacteria and Firmicutes significantly increased after contamination The taxa include Alicyclobacillus, Sphingomonas, Nevskia, and Bacillus. The community is dominated by aromatics or oil-resistant bacteria, a change that is associated with the ecotoxicological effects of crude oils, especially aromatics, at higher concentrations of crude oil , The community can tolerate oil-contaminated environment relatively higher abundance of bacteria.