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[Objective]To establish a method of determining the content of total phenolic acid in Xanthium sibiricum Patrin ex Widder and investigate its anti-inflammatory action. [Methods]Chlorogenic acid was used as reference substance and diluted with 50% methanol solution to constant volume. The absorbancy was determined at wavelength of 426 nm by ultraviolet spectroscopy. With aspirin as positive control,the anti-inflammatory action of Xanthium sibiricum Patrin ex Widder was studied by ear swelling caused by xylene in mice. [Results] The average content of total phenolic acid in Xanthium sibiricum Patrin ex Widder was 5. 22% and the RSD reached 0. 17%. It could significantly relieve ear swelling caused by xylene in mice. Compared with normal saline,the result was of significant difference(P < 0. 01). [Conclusions]The method was convenient and accurate,so it could be used to measure the content of total phenolic acid in Xanthium sibiricum Patrin ex Widder and explore its anti-inflammatory action.
[Objective] To establish a method of determining the content of total phenolic acid in Xanthium sibiricum Patrin ex Widder and investigate its anti-inflammatory action. [Methods] Chlorogenic acid was used as reference substance and diluted with 50% methanol solution to constant volume. The absorbancy was determined at wavelength of 426 nm by ultraviolet spectroscopy. With aspirin as positive control, the anti-inflammatory action of Xanthium sibiricum Patrin ex Widder was studied by ear swelling caused by xylene in mice. [Results] The average content of total phenolic acid in Xanthium sibiricum Patrin ex Widder was 5. 22% and the RSD reached 0. 17%. It could be significantly relieved ear swelling caused by xylene in mice. Compared with normal saline, the result was significant difference (P <0.01 [Conclusions] The method was convenient and accurate, so it could be used to measure the content of total phenolic acid in Xanthium sibiricum Patrin ex Widder and explore its anti-inflammatory acti on.