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目的:对以HPLC法测定菊花中绿原酸含量的测量不确定度进行分析,找出影响不确定度的因素,为评价检测报告提供科学依据。方法:用HPLC法测定菊花中绿原酸含量评估其不确定度,并根据《测量不确定度评定与表示》(JJF1059—1999)中有关规定评估其不确定度。结果:本次实验的不确定度评估为(0.369±0.0338)%。结论:发现本次实验的不确定度主要由样品的处理所引入。
OBJECTIVE: To analyze the uncertainty of determination of chlorogenic acid in chrysanthemum by HPLC and to find out the factors that affect the uncertainty, so as to provide a scientific basis for evaluating the detection report. Methods: The uncertainty of chlorogenic acid content in chrysanthemum was determined by HPLC method and its uncertainty was evaluated according to the relevant regulations of “Evaluation and Expression of Uncertainty in Measurement” (JJF1059-1999). Results: The uncertainty of this experiment was evaluated as (0.369 ± 0.0338)%. Conclusion: The uncertainty of this experiment was found to be mainly caused by the sample processing.