论文部分内容阅读
目的建立L-02细胞中3-羟基-3-甲基戊二酰辅酶A(HMG-Co A)还原酶活性的检测方法。方法色谱柱:C18色谱柱,流动相:甲醇-水(20μmol·L-1磷酸二氢钾,p H 7.2)=6∶94,检测波长:340 nm,柱温:25℃,流速:1m L·min-1。考察该方法的专属性、标准曲线和定量下限、精密度与回收率、稳定性。结果还原型辅酶Ⅱ线性范围为2.5~1000μmol·L-1,定量下限为0.5μmol·L-1,日内、日间RSD分别在(5~6)%和(4~8)%内,相对回收率在(96.97~99.57)%内。结论建立的L-02细胞中HMG-Co A还原酶活性检测方法符合实验要求,且可用于测定氟伐他汀对L-02细胞HMG-Co A还原酶活性的抑制作用。
Objective To establish a method for the determination of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-Co A) reductase activity in L-02 cells. Methods Column: C18 column, mobile phase: methanol-water (20 μmol·L-1 potassium dihydrogen phosphate, p H 7.2) = 6:94, detection wavelength: 340 nm, column temperature: 25 ℃, · Min-1. Investigate the specificity of the method, the standard curve and lower limit of quantification, precision and recovery, stability. Results The linear range of the reduced coenzyme Ⅱ was 2.5-1000 μmol·L -1 and the lower limit of quantification was 0.5 μmol·L -1. The intra-day and inter-day RSDs were relatively recovered within 5-6% and 4-8%, respectively The rate of (96.97 ~ 99.57)%. Conclusion The detection method of HMG-Co A reductase activity in L-02 cells accords with the experimental requirements and can be used to determine the inhibitory effect of fluvastatin on HMG-Co A reductase activity in L-02 cells.