论文部分内容阅读
根据柯萨奇Ｂ组病毒（ＣＢＶ）基因组５’非编码区核苷酸序列，选用了一对组特异性引物，用于逆转录聚合酶链反应检测ＣＢＶ－ＲＮＡ。结果显示该法可检出ＣＢＶ１～６型ＲＮＡ，灵敏度为１０ＰＦＵ，不能检出其他病毒ＲＮＡ。采用的碘化钠－异硫氰酸胍－氯仿法提取ＣＢＶ－ＲＮＡ与传统的蛋白酶Ｋ－酚－氯仿法及异硫氰酸胍－酚－氯仿法比较，具有快速、简便、稳定、可靠的特点。应用该法检测ＣＢＶ－５感染鼠外周血ＲＮＡ，第３ｄ即为阳性，提示本法可用于ＣＢＶ感染的早期诊断
According to the 5 ’non-coding region nucleotide sequence of Coxsackie B virus (CBV) genome, a pair of group-specific primers was selected for reverse transcriptase polymerase chain reaction to detect CBV-RNA. The results showed that this method can detect CBV1 ~ 6 RNA, the sensitivity of 10PFU, can not detect other viral RNA. Compared with traditional protease K-phenol-chloroform method and guanidinium isothiocyanate-phenol-chloroform method, CBV-RNA extracted by sodium iodide-guanidinium isothiocyanate-chloroform method has the advantages of fast, simple, stable and reliable Features. Application of this method to detect CBV-5 infected rat peripheral blood RNA, 3d is positive, suggesting that this method can be used for early diagnosis of CBV infection