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构建了甜菜坏死黄脉病毒（BNYVV）RNA4全长核苷酸序列以及5种编码区突变体的侵染性cDNA克隆.不同结构的RNA4体外转录物与只含有RNA1,2和3的BNYVV分离物总RNAs混合后分别接种寄主植物番杏（Tetragonia expansa）作为毒源,繁殖后接种甜菜,利用无毒甜菜多黏菌（Polymyxa betae）进行传毒实验.结果表明,RNA4编码区内各种缺失突变均能显著地抑制多黏菌的传播,但插入4个碱基的移码突变体对传毒没有影响,说明BNYVV RNA4中的部分核苷酸序列对于被真菌介体高效传播是必需的,并且可能是在RNA水平上具有功能.
The full length nucleotide sequence of beet necrotic vein virus (BNYVV) RNA4 and the infectious cDNA clone of five coding region mutants were constructed.The RNA4 in vitro transcripts of different structures were compared with BNYVV isolates containing only RNA1, 2, and 3 After mixed, the total RNAs were inoculated with the host plant Tetragonia expansa as the source of the virus, which was then inoculated into the beet and propagated using the non-toxic polymyxa betae.The results showed that various deletions Could significantly inhibit the transmission of polymyxa, but the insertion of a 4-base frameshift mutant had no effect on the transmission of the virus, indicating that some of the nucleotide sequences in BNYVV RNA4 are essential for efficient transmission by fungal mediators, and It may be functional at the RNA level.