Phenotypical Reversion and Differentiation of Breast Cancer in vitro by Sodium Phenylacetate

• 作 者: Ji Jiaxiang Lu Zhongmou JI Xia
• 机 构: Department of Microbiology and Immunology, Nanjing Medical University, Nanjing 210029, P. R. China“,
• 出 处: 黑龙江科技学院学报
• 发表日期: 2004年期
• 关键词: sodium phenylacetate breast cancer cells phenotype reversion differentiation

Objective To evaluate the effect of a nontoxic differentiation inducer, sodium phenylacetate (NaPA) on breast cancer MCF-7 and MDA-453 cells in vitro. Methods The anchorage growth assay, ELISA, immunofluorescence analysis and electron microscopy were used to study the effect of NaPA on MCF-7 and MDA-453 cells. Results NaPA treatment resulted in a dose-dependent inhibition of MCF-7 or MDA-453 cell growth, including anchorage-dependent and anchorage-independent growth, but il did not significantly inhibit the growth of normal amnion tissue Wish cells and adult hepatic cells L-02. In addition, NaPA could enhance the expressions of intercellular adhesion molecule-1(ICAM-1), human leukocyte antigen ( HLA ) class Ⅰ and Ⅱ molecules on the surface of MCF-7 and MDA-453 cells. Electron micrographs demonstrated richer rough endoplasmic reticulum,mitochondria in NaPA-treated MCF-7 and MDA-453 cells, in contrast to the scant before-mentwned structures but scattered cytoplasmic polyribosomes in the untreated ones. Conclusion NaPA is effective in inducing phenotype reversion and differentiation of breast cancer, and potential in cancer intervention.