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江南油杉(Keteleeria cyclolepis Flous)又名浙江油杉,产于我国云南、贵州、广西、广东、湖南、江西、浙江等省区。本试验旨在通过对江南油杉染色体核型的观察和分析,期望能为江南油杉的育种工作提供有益的资料。材料与方法取江南油杉种子,先用60℃温水浸泡片刻,再用40℃的水恒温浸泡6小时,然后置25℃恒温下培养,待种子长出1-2厘米长的胚根时,用0.002M8-羟基喹啉水溶液在室温下处理6-8小时,水洗后用3:1的卡诺氏液(95%乙醇3份和冰醋酸1份)固定2-24小时后,放入1NHCl溶液于60℃恒温中解离5分钟左右,切下根尖分生组织用改良的苯酚品红液染色、压
Keteleeria cyclolepis Flous (also known as Zhejiang oleander), produced in our country Yunnan, Guizhou, Guangxi, Guangdong, Hunan, Jiangxi, Zhejiang and other provinces. The purpose of this experiment is to observe and analyze the chromosome karyotypes of Olea europaea and to provide useful data for the breeding of Olea europaea. MATERIALS AND METHODS The seeds of A. niloticus were first immersed in warm water at 60 ° C for a while and then soaked in water at 40 ° C for 6 hours at constant temperature and then incubated at 25 ° C until the radicles grew 1-2 cm in length. Treated with 0.002M 8-hydroxyquinoline aqueous solution at room temperature for 6-8 hours, washed with water and then fixed with 3: 1 Carnot’s solution (3 parts of 95% ethanol and 1 part of glacial acetic acid) for 2-24 hours, and then 1N HCl The solution was dissociated at a constant temperature of 60 ° C for about 5 minutes and the apical meristem was dissected and stained with a modified phenol magenta solution