菌寄生真菌纤细齿梗孢一丝氨酸-苏氨酸蛋白激酶基因OPK1的克隆及序列分析

• 作 者: 赵培宝 任爱芝 李多川
• 机 构: 山东农业大学植物保护学院,聊城大学植物保护系,
• 出 处: 植物病理学报
• 发表日期: 2010年03期
• 关键词: Olpitrichum tenellum protein kinase gene cloning

Using degenerate PCR and TAIL-PCR,a protein kinase gene OPK1(Genebank accession No.:EU417815) was cloned from mycoparasite fungi Olpitrichum tenellum.OPK1 has an open reading frame of 2 031 bp interrupted by two introns(108 bp,84 bp) and putatively encodes a protein of 676 aa.Phylogenetic analysis indicated that OPK1 was most similar to other serine-threonine protein kinase in fungi.Southern blot analysis indicated that OPK1 is present as a single copy in the genome.RT-PCR showed it could be transcribed both in the phase of spore germination and hyphal growth.

Using degenerate PCR and TAIL-PCR, a protein kinase gene OPK1 (Genebank accession No.:U417815) was cloned from mycoparasite fungi Olpitrichum tenellum. OPK1 has an open reading frame of 2 031 bp interrupted by two introns (108 bp, 84 bp) and putatively encodes a protein of 676 aa. Phylogenetic analysis indicated that OPK1 was most similar to other serine-threonine protein kinase in fungi. Southern blot analysis indicates that OPK1 is present as a single copy in the genome. RT-PCR showed it could be transcribed both in the phase of spore germination and hyphal growth.