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目的探讨胆必清颗粒对实验性急性细菌性胆管炎保护作用的机理.方法经胆管内注射1 mL大肠杆菌(1×108cfu@mL)引发急性细菌性胆管炎.采用硝酸还原法测定血清NO水平;酸滴定法测定PLA2活性;放免法测定血清TNF-α,IL6和血浆TXB2和PGFα水平.结果与对照组比较,模型组血清NO、PL2、TNF和血浆TXB2水平均明显升高(P＜0.05);各胆必清治疗组的上述指标均显著降低(P＜0.01).结论胆必清颗粒能明显抑制前炎性因子PLA2和炎性细胞因子TNF-α,IL-6的过量产生,从而进一步揭示了该方剂的抗炎和屏障保护作用机理.“,”Aim To study the mechanism of protective effects of Danbiqing granule (DBQ) on experimental acute bac-terial cholangitis in rabbits. Methods The acute bacterial cholangitis was induced by injecting 1 mL of 1 × 108 cfu@mL-1Escherchia coli suspension into common bile duit. The serum nitrous oxide (NO) levels were measured using nitric acid reductase kit. Phospholipase A2 ( PLA2 ) activity was assayed by a method of acid titration (microassay). Serum tumor necrcsis factor-α (TNF-α), inferleukin-6 (IL-6) and plasma thromboxane B2 ( TXB2 ), 6-keto-platelet growth factor 1 (PGFiα) were determined by radioimmunoassay. Results Compared with control group, serum NO, PLA2, TNF-α, IL-6and plasma TXB2 levels increased significantly in model group ( P ＜ 0.01) while those of DBQ groups decreased significant-ly( P ＜ 0.01). Conclusion DBQ dramatically inhibits the overproduction of pro-inflammatory factor PLA2 and inflammatory cytokine. Hence, the mechanism of DBQ underlying anti-inflammatory and protective effect against acute bacterial cholangi-tis in rabbits has been revealed.