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目的建立骨健口服液中延胡索乙素的含量测定方法。方法采用反相高效液相色谱法。色谱柱:ZORBAX XDB-C18柱(150mm×4.6mm,5μm),流动相:0.1%磷酸溶液(用三乙胺调节pH值至6.0)-甲醇(45∶55),检测波长:280nm,流速:0.8mL·min-1,柱温:45℃。结果延胡索乙素在0.054～2.160μg内线性关系良好,相关系数r=0.9999,平均回收率为98.9%,RSD=0.5%(n=9)。结论该方法简便、准确、重现性好,可用于骨健口服液的质量控制。
Objective To establish a method for the determination of tetrahydropalmatine in Gujian Oral Liquid. Method using reversed-phase high performance liquid chromatography. Column: ZORBAX XDB-C18 column (150mm x 4.6mm, 5μm), mobile phase: 0.1% phosphoric acid solution (pH adjusted to 6.0 with triethylamine) - methanol (45:55), detection wavelength: 280nm, flow rate: 0.8 mL·min-1, column temperature: 45°C. Results The linear relationship of tetrahydropalmatine was good in the range of 0.054 to 2.160 μg. The correlation coefficient r was 0.9999. The average recovery was 98.9%, and RSD was 0.5% (n=9). Conclusion The method is simple, accurate and reproducible. It can be used for the quality control of bone health oral liquid.