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目的比较凋亡调控因子Survivin及caspase-3在自然流产及反复自然流产组患者绒毛中的表达,探讨两者在早期自然流产发生中的作用及相关性。方法随机抽取正常早孕组、首次自然流产组及反复自然流产组患者各30例,应用免疫组织化学SP法检测Survivin与caspase-3在绒毛滋养层细胞中的定位和表达。结果首次自然流产组及反复自然流产组患者绒毛中Survivin的表达均低于正常早孕组,而caspase-3则高于正常早孕组,差异均有显著性统计学意义(P<0.05)。两流产组间患者绒毛中这两者的表达则无统计学意义(P>0.05)。流产组患者绒毛中Survivin与caspase-3呈负相关。结论早期自然流产的发生可能与Survivin表达减少caspase-3表达增加引起滋养层细胞过度凋亡有关。
Objective To compare the expression of Survivin and caspase-3 in the villus of patients with spontaneous abortion and recurrent spontaneous abortion, and to explore their roles in early spontaneous abortion. Methods 30 cases of normal pregnancy group, primary spontaneous abortion group and recurrent spontaneous abortion group were randomly selected. The localization and expression of Survivin and caspase-3 in trophoblast cells were detected by immunohistochemical SP method. Results The expression of Survivin in the first spontaneous abortion group and recurrent spontaneous abortion group was lower than that in the normal early pregnancy group, while the caspase-3 level was higher than that in the normal pregnancy group (P <0.05). There was no significant difference between the two groups (P> 0.05). Survivin and caspase-3 in the villi of abortion group were negatively correlated. Conclusions The occurrence of early spontaneous abortion may be related to the decrease of Survivin expression and the increase of caspase-3 expression leading to excessive apoptosis of trophoblast cells.