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在pH 3 0～ 4 3的酸性介质中 ,铝 铬天青S TritonX 10 0配合物与蛋白质迅速反应生成多元配合物 ,从而引起吸收光谱的改变 ,在 2 2 0nm和 6 36nm附近吸光度增大 ,且吸光度差ΔA(=A0 -A)值与蛋白质的浓度成正比 .不同蛋白质在 0～ 5 0mg/L和 10～ 80mg/L范围内遵循比尔定律 ,各反应的摩尔吸光系数分别在 4 2 3× 10 5～ 2 0 1× 10 6(2 2 0nm附近 )和 2 6 4× 10 5～ 1 6 4× 10 6(6 36nm附近 )之间 .基于此 ,建立了一种测定蛋白质的新光度法 .该法简便、快速、选择性好 ,用于人血清和尿液样品中蛋白质总量的测定 ,结果满意
In pH 3 0 ~ 4 3 acid medium, aluminum chrome azure S TritonX 10 0 complex quickly react with proteins to generate multiple complexes, which led to the change of absorption spectrum. The absorbance increased near 220 nm and 6 36 nm, And the difference of absorbance ΔA (= A0 -A) was proportional to the concentration of protein.The Beer’s law was obeyed in the range of 0 ~ 50 mg / L and 10 ~ 80 mg / L for different proteins, and the molar absorptivities of each reaction were respectively 4 2 3 × 10 5 ~ 2 0 1 × 10 6 (near 220 nm) and 2 6 4 × 10 5 ~ 1 6 4 × 10 6 (near 6 36 nm) .Therefore, a new photometric method for the determination of protein Method.The method is simple, rapid and selective and is suitable for the determination of total protein in human serum and urine samples with satisfactory results