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目的监测2012-2015年黑龙江省诺如病毒腹泻的流行情况及流行株的基因型、重组变异特征。方法 2012年1月-2015年12月共采集480份哨点医院腹泻病人的粪便标本,采用Real-time PCR检测诺如病毒GI和GII基因组,对GII基因组阳性标本经传统RT-PCR扩增诺如病毒部分RNA依赖的RNA聚合酶(Rd Rp)和衣壳蛋白(VP1)序列,对核苷酸序列进行基因分型。结果 2012-2015年采集的腹泻病人粪便标本诺如病毒阳性率为3.54%(17/480),GI阳性1株,GII阳性16株。其中11株GII阳性株扩增测序成功,分为6种基因型,分别为:GII.4 Sydney_2012型、GII.4 Den Haag_2006b型、GII.P7/GII.6型、GII.P12/GII.3型、GII.P16/GII.13型和GII.17型。结论黑龙江省2012-2015年诺如病毒存在多种基因型,并存在不同基因型间重组株,提示诺如病毒在黑龙江省的遗传变异日趋复杂。
Objective To monitor the prevalence of Norovirus diarrhea in Heilongjiang province from 2012 to 2015 and the genotypes and recombination variation characteristics of the epidemic strains. Methods A total of 480 stool specimens from diarrhea patients in sentinel hospital were collected from January 2012 to December 2015. Real-time PCR was used to detect Norovirus GI and GII genomes. The GII genomic positive samples were amplified by RT-PCR Nucleotide sequences are genotyped, for example, viral RNA-dependent RNA polymerase (RdRp) and capsid protein (VP1) sequences. Results The positive rate of Norovirus was 3.54% (17/480) in stool samples of diarrhea patients collected in 2012-2015, one was GI positive and the other was GII positive. Eleven GII positive strains were successfully amplified and sequenced and divided into six genotypes: GII.4 Sydney_2012, GII.4 Den Haag_2006b, GII.P7 / GII.6, GII.P12 / GII.3 Type, GII.P16 / GII.13 type and GII.17 type. Conclusion There are multiple genotypes of norovirus between 2012 and 2015 in Heilongjiang Province. Recombinant strains between different genotypes exist, suggesting that the genetic variation of Norovirus in Heilongjiang Province is becoming more and more complicated.