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目的:探讨还少丹对衰老模型小鼠白介素-2(IL-2)及线粒体DNA(mitochondrial DNA,mtDNA)含量的影响。方法:将40只小鼠随机分成空白对照组、衰老模型组、低剂量药物组和高剂量药物组,每组10只。采用皮下注射D-半乳糖溶液(100 mg/kg)制备亚急性衰老模型,同时用还少丹水煎液灌胃6周。双抗夹心ELISA法测定血清IL-2的含量;分光光度法测定肝、脑组织中mtDNA含量。结果:与空白对照组比较,模型组小鼠血清IL-2含量明显减少,肝和脑组织mtDNA含量明显增高(均P<0.01)。与模型组比较,还少丹低剂量和高剂量组都能提高衰老模型小鼠血清IL-2的含量,降低肝、脑组织mtDNA的含量,差异均具有统计学意义(均P<0.01)。结论:还少丹能够提高衰老小鼠的机体免疫功能,降低肝、脑组织中mtDNA的含量,从而起到延缓衰老的作用。
Objective: To investigate the effect of Hsudansan on interleukin-2 (IL-2) and mitochondrial DNA (mtDNA) in aging mice. Methods: Forty mice were randomly divided into blank control group, aging model group, low dose drug group and high dose drug group, with 10 mice in each group. A sub-acute aging model was established by subcutaneous injection of D-galactose solution (100 mg / kg), while gavage was performed for 6 weeks with Shao Dan decoction. The content of IL-2 in serum was detected by double-antibody sandwich ELISA. The content of mtDNA in liver and brain was determined by spectrophotometry. Results: Compared with the blank control group, the content of IL-2 in the model group decreased significantly and the contents of mtDNA in liver and brain tissue were significantly increased (all P <0.01). Compared with the model group, Shaodan low-dose and high-dose groups could both increase the content of IL-2 in serum and the content of mtDNA in liver and brain tissue in aging model mice (all P <0.01). Conclusion: Shaotan Dan also can improve the immune function of aging mice, reduce the content of mtDNA in liver and brain tissue, and thus play a role in anti-aging.