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用妊娠第14天大鼠胚胎中脑神经细胞作微团培养,培养物接触维生素A(VitA)和3-甲基胆蒽(3-MCA)后,微团中的集落形成率明显降低,半数分化抑制浓度分别为10ng/ml和1.0ng/ml,有明显的致畸性。在培养12小时内,还原型谷胱甘肽(GSH)和谷胱甘肽S-转移酶(GST)水平明显降低,24小时后逐步快复正常,而GST活性继续升高,于48小时达最大值。这两种化学物的体外致畸作用可能与GSH耗竭有一定关系。
After 14 days of gestation, rat embryo midbrain nerve cells were cultured as micelles. After the cultures were exposed to VitA and 3-MCA, the colony formation rate in the micelles was significantly reduced. Half of the cultures Differentiation inhibitory concentrations were 10ng / ml and 1.0ng / ml, with significant teratogenicity. Within 12 hours of incubation, the level of GSH and GST decreased significantly. After 24 hours, the levels of GSH and GST gradually increased and returned to normal, while the activity of GST continued to rise at 48 hours Maximum In vitro teratogenic effects of these two chemicals may be related to the depletion of GSH.