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近年来,开展了以发酵罐代替蘑菇瓶制备液体菌种的研究,但至今尚未见到低本而高效的代用配方报导。我们通过一年的研究,已能使摇瓶中每毫升的发酵液菌球数达到800～1000,每斤发酵液成本只须九厘八毫五。现简报如下: (一)材料方法:菌种为本所3号种。培养基原料有玉米粉、菜饼、酵母粉、钾、镁及B_1等。将菜饼加5.5倍水,用HCl调pH至1.5左右,表压控制1.2kg/cm~2水解1小时。用100目滁纶布过滤,然后取配方所需
In recent years, research has been carried out on the use of fermenters instead of mushroom bottles for the preparation of liquid bacterial strains, but so far no low-cost and efficient alternative formulation has been reported. Through a year of research, we have been able to shake the flask per ml of fermented mycelium number 800 ~ 1000, per kilogram of fermentation broth cost only 90%. Now briefings are as follows: (a) material methods: strain of the No. 3 species. Medium raw materials are corn flour, vegetable cake, yeast powder, potassium, magnesium and B_1 and so on. The cake plus 5.5 times the water, pH adjusted to about 1.5 with HCl, gauge pressure control 1.2kg / cm ~ 2 hydrolysis 1 hour. With 100 mesh Chu Lun cloth filter, and then take the formula required