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This study reported that the hapten of the organophosphorus insecticide chlorpyrifos, O,O-diethyl-O-[3,5-dichloro-6-(2-carboxyethyl)thio-2-pyridyl]phosphorothioate(named AR) wassynthesized by using technical grade chlorpyrifos reacted with 3-marcapropanoic acid in hotalkaline solution. The hapten was conjugated to bovine serum albumin (BSA) with the modifiedactive ester method to form artificial immune antigen. The ratio of AR:BSA was 39:1. Theartificial coating antigen for chlorpyrifos was synthesized by conjugating AR to ovalbumin(OVA) with the mixed-anhydride method, and the ratio was 13:1. The anti-chlorpyrifos polyclonalantibodies were obtained by using the artificial immune antigen (AR-BSA) to immune in therabbits.
This study reported that the hapten of the organophosphorus insecticide chlorpyrifos, O, O-diethyl-O- [3,5-dichloro-6- (2-carboxyethyl) thio-2-pyridyl] The hapten was conjugated to bovine serum albumin (BSA) with the modified active ester method to form artificial immune antigen. The ratio of AR: BSA was 39: 1. The artificial coating antigen for chlorpyrifos was synthesized by conjugating AR to ovalbumin (OVA) with the mixed-anhydride method, and the ratio was 13: 1. The anti-chlorpyrifos polyclonalantibodies were obtained by using the artificial immune antigen (AR- BSA) to immune in therabbits.