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目的 :克隆变形链球菌葡聚糖结合蛋白B(GbpB)功能区的基因片段 ,并在大肠杆菌中表达。方法 :通过PCR技术克隆GbpB功能区的基因片段并通过蛋白重组融合表达技术使其在大肠杆菌中表达。 结果 :成功克隆了GbpB功能区的基因片段 ,并在大肠杆菌中得到其融合蛋白的表达。 结论 :利用分子生物学技术能够成功克隆GbpB功能区基因并获得其融合蛋白的表达 ,为后续研究奠定了基础
Objective: To clone the gene fragment of Streptococcus mutans dextran binding protein B (GbpB) and express it in Escherichia coli. Methods: The gene fragment of GbpB functional region was cloned by PCR and expressed in E. coli by recombinant fusion technique. Results: The gene fragment of GbpB functional region was successfully cloned and the expression of its fusion protein was obtained in E. coli. Conclusion: The successful cloning of GbpB functional gene and its expression of fusion protein using molecular biology techniques laid the foundation for further study