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等电聚焦是六十年代中期发展起来的一项新技术,它可提高凝胶电泳的分辩率,一般用聚丙烯酰胺凝胶电泳区分不清的同工酶谱带,用此技术可区分清楚,并用可见光扫描图谱进行对照,能提高分辩率。用此技术在真菌同工酶方面的研究,国内未见报道,本文用等电聚焦技术对真菌斗菇属的香菇和侧耳属的平菇进行了酯酶和过氧化物酶同工酶的研究。一、供试材料(一)菌种香菇B_1、B_2、85-63F、8001由南京野生植物试验场提供;平菇C_(25)、C_(26)由
Isoelectric focusing is a new technology developed in the mid-1960s. It can improve the resolution of gel electrophoresis. Generally, isozyme bands separated by polyacrylamide gel electrophoresis can be distinguished clearly by this technique , And compared with the visible light scan spectrum, can improve the resolution. With this technology in the field of fungal isozyme research, no reports in the country, this paper by the use of isoelectric focusing fungus mushroom genus Mushroom and Pleurotus ostreatus were esterase and peroxidase isozymes . First, the test materials (A) Mushrooms mushrooms B_1, B_2, 85-63F, 8001 provided by the Nanjing wild plant test site; Pleurotus ostreatus C_ (25), C_ (26)