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[目的]研究快速建立模拟临床过程的小鼠结肠癌原位移植瘤及肝转移模型方法。[方法]采用小鼠结肠癌细胞CT26接种于裸鼠皮下,形成稳定传代的皮下种植瘤。再取该肿瘤组织块,用原位医用胶黏合法种植于BALB/c小鼠盲肠壁,建立模拟于临床的结肠癌肝转移模型,定时观察小鼠全身情况,分不同时期处死,观察其生长及转移特性。[结果]原位种植成瘤率100%,术后3周肝转移率100%,4周后动物消瘦,萎靡,行动迟缓,反应迟钝,部分可见腹水形成,濒临死亡。中位生存期29d。[结论]小鼠结肠癌完整组织块通过“生物胶黏贴法”原位种植BALB/c小鼠盲肠壁,速度快,方法简便,能较好的重现结肠癌临床转移的过程,为人类结肠癌生长、肝转移机制及干预肝转移治疗的研究提供一种较理想的动物模型。
[Objective] To study the method of rapidly establishing orthotopic colon carcinoma and liver metastasis model of mouse colon cancer by simulating clinical process. [Method] The colon cancer cell line CT26 was inoculated subcutaneously in nude mice to form stable subculture subcutaneous tumor. The tumor tissue pieces were harvested and implanted in the cecal wall of BALB / c mice with the in-situ medical adhesive method. The model of liver metastasis of colon cancer was established, and the whole body condition of the mice was observed regularly. The mice were sacrificed at different time points to observe their growth And transfer characteristics. [Result] The rate of in-situ implantation was 100%. The rate of liver metastasis was 100% after 3 weeks of operation. After 4 weeks, the animals became wasting, apathetic, slow in action and unresponsive. Some ascites were formed and dying. The median survival 29d. [Conclusion] The colon tissue of mice with colon cancer can be in situ grown on the cecal wall of BALB / c mice via “bio-adhesive” method. The method is simple and can reproduce the clinical metastasis of colon cancer. It provides an ideal animal model for the study of human colon cancer growth, liver metastasis mechanism and interventional liver metastasis.