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为查明吸血期蓖麻硬蜱雌虫体内周身性莱姆病螺旋体生长动态，用直接免疫荧光试验、银染色组织学及电子显微镜技术，对饥饿及吸血期蓖麻硬蜱雌虫脑、唾液腺和卵巢组织中莱姆病螺旋体的分布和数量进行了观察。结果显示，饥饿蟀组织中螺旋体数量极为有限，吸血期蜱组织中螺旋体的密度和数量随吸血时间的延长而快速增长，至开始吸血后4或5天，脑、唾液腺和卵巢组织中螺旋体的数量都已达到无法计数的程度；本研究首次对周身性莱姆病螺旋体的分裂增殖提出了假设。螺旋体在吸血期蜱唾液腺的大量检出，为莱姆病螺旋体经由蜱唾液分泌而传播给脊椎动物宿主的假设提供了新的证据。
In order to find out the dynamics of Lyme disease in vivo during the sucking period, the direct and indirect immunofluorescence assay, silver staining histology and electron microscopy were used to study the effects of starvation and sucking period on the brain, salivary gland And the distribution and number of Lyme disease spirochetes in ovarian tissue were observed. The results showed that the number of spirochetes in starving crickets was extremely limited. The density and number of spirochetes in blood-sucking ticks rapidly increased with the increase of blood-sucking time. The number of spirochetes in brain, salivary gland and ovary increased to 4 or 5 days after the start of blood-sucking Have reached the level that can not be counted; for the first time, this study made the hypothesis on the division and proliferation of whole body Lyme disease spirochetes. The large detection of spirochetes in the tick salivary glands during the bloodsucking period provides new evidence for the hypothesis that Lyme disease spirochetes are transmitted to vertebrate hosts via tick salivary secretion.