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目的:探讨右丙亚胺(DEX)对表柔比星(EPI)诱导乳腺癌细胞凋亡的影响。方法:分别以不同浓度EPI作用于MCF-7、MDA-MB-231和BT474 3种乳腺癌细胞系24h,用MTT法检测抑制率,选择EPI最佳实验浓度;应用1.0μg/mL EPI与1.0、10和20μg/mL DEX分别及联合作用于3种乳腺癌细胞系,用MTT法检测抑制率,用流式细胞术检测细胞凋亡。结果:1.0μg/mL EPI组3种乳腺癌细胞的抑制率分别为(48.72±0.34)%、(64.40±2.63)%和(58.57±2.23)%,1.0μg/mL EPI联合1.0μg/mL DEX组3种细胞的抑制率分别为(46.44±1.35)%、(65.20±0.21)%和(57.52±2.31)%,1.0μg/mL EPI联合10μg/mL DEX组3种细胞抑制率分别为(47.57±2.20)%、(61.70±1.08)%和(59.54±1.26)%,1.0μg/mL EPI联合20μg/mL DEX组3种细胞抑制率分别为(43.85±1.19)%、(60.80±0.62)%和(58.25±3.50)%,EPI和DEX两药联合组(EPI∶DEX=1∶1和1∶10)与EPI组3种乳腺癌细胞系的抑制率比较差异无统计学意义,P>0.05;两者浓度为1∶20时对3种乳腺癌细胞系的增殖影响差异有统计学意义,P<0.05。流式细胞术检测1.0μg/mL EPI组3种乳腺癌细胞凋亡率分别为8.54%、11.25%和10.78%,EPI∶DEX=1∶10组3种乳腺癌细胞凋亡率分别为8.74%、11.85%和10.49%,两组细胞凋亡率差异无统计学意义,P>0.05。结论:心脏保护剂量的DEX并不影响EPI诱导的乳腺癌细胞凋亡效果。
Objective: To investigate the effect of dexmedetomidine (DEX) on apoptosis of breast cancer cells induced by epirubicin (EPI). Methods: The three kinds of breast cancer cell lines MCF-7, MDA-MB-231 and BT474 were treated with EPI at different concentrations for 24 h respectively. The inhibitory rates were determined by MTT assay and the optimal concentration of EPI was selected. , 10 and 20μg / mL DEX respectively and in combination on three kinds of breast cancer cell lines. The inhibition rate was detected by MTT assay and the apoptosis was detected by flow cytometry. Results: The inhibitory rates of three kinds of breast cancer cells in the 1.0μg / mL EPI group were (48.72 ± 0.34)%, (64.40 ± 2.63)% and (58.57 ± 2.23)%, (46.44 ± 1.35)%, (65.20 ± 0.21)% and (57.52 ± 2.31)%, respectively. The inhibitory rates of the three kinds of cells in the three groups were (47.57 (43.85 ± 1.19)% and (60.80 ± 0.62)%, respectively (P <0.05). The inhibitory rates of these two cell lines were significantly different from those in the DEX group (± 2.20%, 61.70 ± 1.08% and 59.54 ± 1.26% And (58.25 ± 3.50)%, respectively. There was no significant difference in the inhibition rates between EPI and DEX (EPI: DEX = 1: 1 and 1:10) ; When the two concentrations were 1:20, the proliferation of three kinds of breast cancer cell lines had significant difference (P <0.05). Flow cytometry showed that the apoptotic rates of three kinds of breast cancer cells in the 1.0μg / mL EPI group were 8.54%, 11.25% and 10.78%, respectively. The apoptosis rates of three kinds of breast cancer cells in EPI: DEX = 1:10 group were 8.74% , 11.85% and 10.49%, respectively. There was no significant difference in apoptosis rate between the two groups (P> 0.05). Conclusion: Cardioprotective doses of DEX do not affect EPI-induced breast cancer cell apoptosis.