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AIM:To refine the loss of heterozygosity on chromosome5p15 and to identify the new tumor suppressor gene (s) incolorectal tumorigenesis.METHODS:Sixteen polymorphic microsatellite markerswere analyzed on chromosome 5 and another 6 markerswere applied on chromosome 5p15 in 83 cases ofcolorectal and normal DNA by PCR.PCR products wereelectrophoresed on an ABI 377 DNA sequencer.Genescan3.1 and Genotype 2.1 software were used for LOH scanningand analysis.RESULTS:We observed 2 distinct regions of frequent allelicdeletions on Chromosome 5,at D5S416 on 5p15 andDSS428-DSS410 on 5q.Another 6 polymorphric microsatellitemarkers were applied to 5p15 and the minimal region offrequent loss of heterozygosity was established on 5p15spanning the D5S416 locus.CONCLUSION: Through our detailed deletion mapping studies, we have found a critical and precise location of 5p deletions, 5pl5.2-5pl5.3, which must contain one or more unknown tumor suppressor gene (s) of colorectal cancer.
AIM: To refine the loss of heterozygosity on chromosome 5p15 and to identify the new tumor suppressor gene (s) incolorectal tumorigenesis. METHODS: Sixteen polymorphic microsatellite markerswere analyzed on chromosome 5 and another 6 markerswere applied on chromosome 5p15 in 83 cases ofcolorectal and normal DNA by PCR.PC products wereelectrophoresed on an ABI 377 DNA sequencer. Genescan 3.1 and Genotype 2.1 software were used for LOH scanning and analysis. RESULTS: We observed 2 distinct regions of frequent allelic events on Chromosome 5, at D5S416 on 5p15 and DSS428-DSS410 on 5q. Another 6 polymorphic microsatellite markers were applied to 5p15 and the minimal region offrequent loss of heterozygosity was established on 5p15spanning the D5S416 locus. CONCLUSION: Through our detailed deletion mapping studies, we have found a critical and precise location of 5 deletions, 5pl5.2-5pl5 .3, which must contain one or more unknown tumor suppressor gene (s) of colorectal cancer.