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目的:研究野木瓜注射液对背根神经节细胞辣椒素受体通道电流的影响,分析野木瓜注射液阻滞神经传导、产生镇痛作用的机制。方法:在急性分离的大鼠背根神经节细胞膜上,进行全细胞膜片钳记录,观察1%、5%、10%、15%、25%、50%、75%和80%的野木瓜注射液对辣椒素诱发的辣椒素受体通道电流的影响。在施加和不施加野木瓜注射液两种情形下,将辣椒素诱发的TRPV1电流标准化,并作为辣椒素剂量的函数,绘制了辣椒素的量效关系曲线;并利用辣椒素致痛模型来观察野木瓜注射液对在体动物疼痛的影响。结果:野木瓜注射液浓度依赖地抑制背根神经节细胞辣椒素受体通道电流峰值,且野木瓜注射液的拮抗作用能通过增高辣椒素溶液的浓度而被克服,其拮抗TRPV1的方式是竞争性的。在体动物实验显示随着野木瓜浓度增加,小鼠添足时间下降。结论:野木瓜注射可以明显的抑制辣椒素诱发的受体电流,影响痛觉信息中枢传入,这可能是野木瓜产生镇痛作用机制之一。
Objective: To study the effect of wild papaya injection on the current of capsaicin receptor channel in dorsal root ganglion cells, and analyze the mechanism of action of wild papaya injection in blocking nerve conduction and producing analgesic effect. METHODS: Whole-cell patch-clamp recordings were performed on acutely isolated rat dorsal root ganglion cell membranes and injection of wild papaya at 1%, 5%, 10%, 15%, 25%, 50%, 75%, and 80% Effect of liquid on capsaicin - induced capsaicin receptor channel current. The plasma capsaicin-induced TRPV1 currents were normalized with and without wild papaya injection, and the dose-response curve of capsaicin was plotted as a function of capsaicin dose; capsaicin induced pain models were used to observe Effect of Wild Papaya Injection on Pain in Animals. Results: Wild papaya injection inhibited the peak current of capsaicin receptor channel in dorsal root ganglion neurons in a concentration-dependent manner, and the antagonistic effect of wild papaya injection was overcome by increasing the concentration of capsaicin solution. The antagonism of TRPV1 by competition was Sexual. In vivo animal experiments show that with the increase of papaya concentration, the mouse Tim adequate time decreased. Conclusion: Injection of wild papaya can significantly inhibit capsaicin-induced receptor currents, affecting the affective information center, which may be one of the mechanisms of analgesic effect of wild papaya.