Optimization of Extraction Process of Total Coumarins from Tibetan Herb Artemisia Subdigitata Mattf.

• 作 者: Mengjie YU Zhengming YANG Yun TENG Shaoshan ZHANG Zhongjie HUANG Yuan LIU
• 机 构: College of Pharmacy,Southwest University for Nationalities,Ethnic Medicine Institute,Southwest Unive
• 出 处: Medicinal Plant
• 发表日期: 2016年Z5期
• 关键词: Artemisia Tibetan coumarin ultrasonic ethanol optimize guidance convenient filte

[Objectives]To optimize the ultrasonic extraction conditions of total coumarins from Tibetan herb Artemisia subdigitata Mattf.[Methods]In single-factor tests,the effects of ethanol concentration,extraction time,extraction temperature,solid-liquid ratio and ultrasonic time in extraction procedure on the extraction rate of total coumarins in A.subdigitata Mattf. were researched,and the Box-Behnken experimental design was used to optimize the extraction of total coumarins in A.subdigitata Mattf. [Results]The optimum extraction conditions were as follows:53% ethanol,extraction time 30 min,extraction temperature 85 ℃,ratio of solid to liquid 1:52(g/mL) and ultrasonic time 20 min. Under the conditions,the average extraction yield of total coumarin reached 20.01 mg/g. [Conclusions]he optimized method was convenient and feasible,which could provide reference for the extraction of total coumarins from A.subdigitata Mattf.,and provide some guidance and basis for the further exploitation of A.subdigitata Mattf.

[Objectives] To optimize the ultrasonic extraction conditions of total coumarins from Tibetan herb Artemisia subdigitata Mattf. [Methods] In single-factor tests, the effects of ethanol concentration, extraction time, extraction temperature, solid-liquid ratio and ultrasonic time in extraction procedure on the extraction rate of total coumarins in A.subdigitata Mattf. were researched, and the Box-Behnken experimental design was used to optimize the extraction of total coumarins in A.subdigitata Mattf. [Results] The optimum extraction conditions were as follows: 53 % ethanol, extraction time 30 min, extraction temperature 85 ° C, ratio of solid to liquid 1:52 (g / mL) and ultrasonic time 20 min. Under the conditions, the average extraction yield of total coumarin reached 20.01 mg / g. Conclusions] he optimized method was convenient and feasible, which could provide reference for the extraction of total coumarins from A.subdigitata Mattf., And provide some guidance and basis for the further exploitation of As ubdigitata Mattf.